HUNDREDS of Brisbane bus drivers are set to walk off the job this afternoon during peak hour after last-ditch efforts to head off the strike failed.

Rail, Tram and Bus Union members plan to take industrial action between 2:00pm and 6:00pm over pay and conditions

The union and the Brisbane City Council failed to resolve their pay and conditions dispute during a meeting on Thursday evening that lasted for more than six hours.

The council reinstated its offer of a 2.5 per cent pay rise each year for three years, plus a $400 sign-up bonus, but insisted that union control of rostering was non-negotiable.

Union spokesman Tom Brown said he would have liked to see an agreement.

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